Zfp64 participates in Notch signaling and regulates differentiation in mesenchymal cells | Zendy

Kei Sakamoto | Zendy; Yoshihiro Tamamura | Zendy; Kenichi Katsube | Zendy; Akira Yamaguchi | Zendy

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Zfp64 participates in Notch signaling and regulates differentiation in mesenchymal cells

Author(s) -

Kei Sakamoto,

Yoshihiro Tamamura,

Kenichi Katsube,

Akira Yamaguchi

Publication year - 2008

Publication title -

journal of cell science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.384

H-Index - 278

eISSN - 1477-9137

pISSN - 0021-9533

DOI - 10.1242/jcs.023119

Subject(s) - hes1 , biology , notch signaling pathway , transactivation , microbiology and biotechnology , mesenchymal stem cell , runx2 , coactivator , cellular differentiation , lim domain , signal transduction , transcription factor , zinc finger , gene , genetics

Notch signaling is required for multiple aspects of tissue and cell differentiation. In this study, we identified zinc finger protein 64 (Zfp64) as a novel coactivator of Notch1. Zfp64 is associated with the intracellular domain of Notch1, recruited to the promoters of the Notch target genes Hes1 and Hey1, and transactivates them. Zfp64 expression is under the control of Runx2, and is upregulated by direct transactivation of its promoter. Zfp64 suppresses the myogenic differentiation of C2C12 cells and promotes their osteoblastic differentiation. Our data demonstrate two functions of Zfp64: (1) it is a downstream target of Runx2 and, (2) its cognate protein acts as a coactivator of Notch1, which suggests that Zfp64 mediates mesenchymal cell differentiation by modulating Notch signaling.

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