Open AccessIce2p is important for the distribution and structure of the cortical ER network in Saccharomyces cerevisiae
Author(s) -
Paula Estrada de Martin,
Yunrui Du,
Peter Novick,
Susan FerroNovick
Publication year - 2005
Publication title -
journal of cell science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.384
H-Index - 278
eISSN - 1477-9137
pISSN - 0021-9533
DOI - 10.1242/jcs.01583
Subject(s) - biology , endoplasmic reticulum , saccharomyces cerevisiae , microbiology and biotechnology , live cell imaging , cell fractionation , subcellular localization , budding , cytosol , cell , nucleus , transmembrane protein , cell division , yeast , cytoplasm , biochemistry , membrane , receptor , enzyme
In Saccharomyces cerevisiae, the endoplasmic reticulum (ER) is found along the cell periphery (cortical ER) and nucleus (perinuclear ER). In this study, we characterize a novel ER protein called Ice2p that localizes to the cortical and perinuclear ER. Ice2p is predicted to be a type-III transmembrane protein. Cells carrying a genomic disruption of ICE2 display defects in the distribution of cortical ER in mother and daughter cells. Furthermore, fluorescence imaging of ice2Δ cells reveals an abnormal cortical ER tubular network morphology in both the mother cell and the developing bud. Subcellular fractionation analysis using sucrose gradients corroborate the data from the fluorescence studies. Our findings indicate that Ice2p plays a role in forming and/or maintaining the cortical ER network in budding yeast.