Open AccessmRBPome capture identifies the RNA binding protein TRIM71, an essential regulator of spermatogonial differentiation
Author(s) -
Guihua Du,
Xinrui Wang,
Mengcheng Luo,
Weiya Xu,
Tao Zhou,
Mei Wang,
Luping Yu,
Lufan Li,
Lie Cai,
P. Jeremy Wang,
John Li,
Jon M. Oatley,
Xin Wu
Publication year - 2020
Publication title -
development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.754
H-Index - 325
eISSN - 1477-9129
pISSN - 0950-1991
DOI - 10.1242/dev.184655
Subject(s) - biology , rna binding protein , germline , population , somatic cell , progenitor cell , microbiology and biotechnology , stem cell , progenitor , regulator , polyadenylation , proteomics , rna , developmental biology , genetics , gene , demography , sociology
Continual spermatogenesis relies on the actions of an undifferentiated spermatogonial population that is composed of stem cells and progenitors. Here, using mouse models, we explored the role of RNA binding proteins (RBPs) in regulation of the biological activities of this population. Proteins bound to polyadenylated RNAs in primary cultures of undifferentiated spermatogonia were captured with oligo (dT)-conjugated beads after UV-crosslinking and profiled by proteomics (termed as mRBPome capture), yielding a putative repertoire of 473 RBPs. From this database, the RBP TRIM71 was identified and found to be expressed by stem and progenitor spermatogonia in prepubertal and adult mouse testes. Tissue-specific deletion of TRIM71 in the male germline led to reduction of the undifferentiated spermatogonial population and a block in transition to the differentiating state. Collectively, these findings demonstrate a key role of the RBP system in regulation of the spermatogenic lineage and may provide clues about influence on the biology of progenitor cell populations in other lineages.