A vertebrate specific and essential role for sp7/osterix in osteogenesis revealed by gene knock-out in the teleost medaka | Zendy

Tingsheng Yu | Zendy; Martin Graf | Zendy; Joerg Renn | Zendy; Manfred Schartl | Zendy; Daria Larionova | Zendy; Ann Huysseune | Zendy; P. Eckhard Witten | Zendy; Christoph Winkler | Zendy

Open Access

A vertebrate specific and essential role for sp7/osterix in osteogenesis revealed by gene knock-out in the teleost medaka

Author(s) -

Tingsheng Yu,

Martin Graf,

Joerg Renn,

Manfred Schartl,

Daria Larionova,

Ann Huysseune,

P. Eckhard Witten,

Christoph Winkler

Publication year - 2016

Publication title -

development

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.754

H-Index - 325

eISSN - 1477-9129

pISSN - 0950-1991

DOI - 10.1242/dev.139550

Subject(s) - biology , intramembranous ossification , vertebrate , microbiology and biotechnology , transcription factor , osteoblast , notochord , zinc finger , ossification , zinc finger transcription factor , phenotype , gene , anatomy , genetics , embryo , embryogenesis , in vitro

osterix (osx; sp7) encodes a zinc-finger transcription factor that controls osteoblast differentiation in mammals. Although identified in all vertebrate lineages, its role in non-mammalian bone formation remains elusive. Here, we show that an osx mutation in medaka results in severe bone defects and larval lethality. Pre-osteoblasts fail to differentiate leading to severe intramembranous and perichondral ossification defects. The notochord sheath mineralizes normally, supporting the idea of an osteoblast-independent mechanism for teleost vertebral centra formation. This study establishes a key role for Osx for bone formation in a non-mammalian species, and reveals conserved and non-conserved features in vertebrate bone formation.

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