Evidence in the mouse for self-renewing stem cells in the formation of a segmented longitudinal structure, the myotome | Zendy

Jean–François Nicolas | Zendy; Luc Mathis | Zendy; Claire Bonnerot | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Evidence in the mouse for self-renewing stem cells in the formation of a segmented longitudinal structure, the myotome

Author(s) -

Jean–François Nicolas,

Luc Mathis,

Claire Bonnerot

Publication year - 1996

Publication title -

development

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.754

H-Index - 325

eISSN - 1477-9129

pISSN - 0950-1991

DOI - 10.1242/dev.122.9.2933

Subject(s) - myotome , biology , microbiology and biotechnology , embryo , progenitor cell , transgene , population , homologous chromosome , genetically modified mouse , anatomy , genetics , gene , embryogenesis , somite , stem cell , demography , sociology

A novel method of clonal analysis has been used in the mouse to define the cellular events that lead to the formation of a segmented longitudinal structure, the myotome. Progenitor cells of the myotome were randomly marked during development by intragenic homologous recombination in transgenic mice expressing a reporter laacZ gene. 153 clones corresponding to 7829 cells, that is 20% of the myotomal population of one embryo, were obtained from 3000 E11.5 embryos. Their analysis leads to the hypothesis that, at E11.5, the 41 segments of the myotome have been mainly produced from a unique, spatially organised pool of self-renewing stem cells that accompanies the formation of the anterior-posterior axis.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research