Making designer mutants in model organisms | Zendy

Ying Peng | Zendy; Karl J. Clark | Zendy; Jarryd M. Campbell | Zendy; Magdalena R. Panetta | Zendy; Yi Guo | Zendy; Stephen C. Ekker | Zendy

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Making designer mutants in model organisms

Author(s) -

Ying Peng,

Karl J. Clark,

Jarryd M. Campbell,

Magdalena R. Panetta,

Yi Guo,

Stephen C. Ekker

Publication year - 2014

Publication title -

development

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.754

H-Index - 325

eISSN - 1477-9129

pISSN - 0950-1991

DOI - 10.1242/dev.102186

Subject(s) - biology , crispr , genome engineering , transcription activator like effector nuclease , genome editing , cas9 , computational biology , zinc finger nuclease , synthetic biology , genome , model organism , genetics , gene

Recent advances in the targeted modification of complex eukaryotic genomes have unlocked a new era of genome engineering. From the pioneering work using zinc-finger nucleases (ZFNs), to the advent of the versatile and specific TALEN systems, and most recently the highly accessible CRISPR/Cas9 systems, we now possess an unprecedented ability to analyze developmental processes using sophisticated designer genetic tools. In this Review, we summarize the common approaches and applications of these still-evolving tools as they are being used in the most popular model developmental systems. Excitingly, these robust and simple genomic engineering tools also promise to revolutionize developmental studies using less well established experimental organisms.

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