Regulation of cranial morphogenesis and cell fate at the neural crest-mesoderm boundary by engrailed 1 | Zendy

Ron A. Deckelbaum | Zendy; Greg Holmes | Zendy; Zhicheng Zhao | Zendy; Chunxiang Tong | Zendy; Claudio Basilico | Zendy; Cynthia A. Loomis | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Regulation of cranial morphogenesis and cell fate at the neural crest-mesoderm boundary by engrailed 1

Author(s) -

Ron A. Deckelbaum,

Greg Holmes,

Zhicheng Zhao,

Chunxiang Tong,

Claudio Basilico,

Cynthia A. Loomis

Publication year - 2012

Publication title -

development

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.754

H-Index - 325

eISSN - 1477-9129

pISSN - 0950-1991

DOI - 10.1242/dev.076729

Subject(s) - biology , mesoderm , mesenchyme , neural crest , microbiology and biotechnology , fate mapping , engrailed , anatomy , cranial neural crest , fibroblast growth factor , coronal suture , mesenchymal stem cell , ingression , progenitor cell , stem cell , genetics , homeobox , skull , embryogenesis , embryonic stem cell , embryo , gastrulation , receptor , gene expression , gene

The characterization of mesenchymal progenitors is central to understanding development, postnatal pathology and evolutionary adaptability. The precise identity of the mesenchymal precursors that generate the coronal suture, an important structural boundary in mammalian skull development, remains unclear. We show in mouse that coronal suture progenitors originate from hedgehog-responsive cephalic paraxial mesoderm (Mes) cells, which migrate rapidly to a supraorbital domain and establish a unidirectional lineage boundary with neural crest (NeuC) mesenchyme. Lineage tracing reveals clonal and stereotypical expansion of supraorbital mesenchymal cells to form the coronal suture between E11.0 and E13.5. We identify engrailed 1 (En1) as a necessary regulator of cell movement and NeuC/Mes lineage boundary positioning during coronal suture formation. In addition, we provide genetic evidence that En1 functions upstream of fibroblast growth factor receptor 2 (Fgfr2) in regulating early calvarial osteogenic differentiation, and postulate that it plays an additional role in precluding premature osteogenic conversion of the sutural mesenchyme.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research