Evidence for a role of vertebrate Disp1 in long-range Shh signaling | Zendy

Letitiah Etheridge | Zendy; Timothy Q. Crawford | Zendy; Shile Zhang | Zendy; Henk Roelink | Zendy

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Evidence for a role of vertebrate Disp1 in long-range Shh signaling

Author(s) -

Letitiah Etheridge,

Timothy Q. Crawford,

Shile Zhang,

Henk Roelink

Publication year - 2009

Publication title -

development

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.754

H-Index - 325

eISSN - 1477-9129

pISSN - 0950-1991

DOI - 10.1242/dev.043547

Subject(s) - biology , sonic hedgehog , microbiology and biotechnology , hedgehog signaling pathway , transmembrane protein , mesoderm , secretion , embryonic stem cell , precursor cell , embryoid body , hedgehog , signal transduction , cell , genetics , gene , endocrinology , receptor , adult stem cell

Dispatched 1 (Disp1) encodes a twelve transmembrane domain protein that is required for long-range sonic hedgehog (Shh) signaling. Inhibition of Disp1 function, both by RNAi or dominant-negative constructs, prevents secretion and results in the accumulation of Shh in source cells. Measuring the Shh response in neuralized embryoid bodies (EBs) derived from embryonic stem (ES) cells, with or without Disp1 function, demonstrates an additional role for Disp1 in cells transporting Shh. Co-cultures with Shh-expressing cells revealed a significant reduction in the range of the contact-dependent Shh response in Disp1(-/-) neuralized EBs. These observations support a dual role for Disp1, not only in the secretion of Shh from the source cells, but also in the subsequent transport of Shh through tissue.

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