Selective plane illumination microscopy techniques in developmental biology | Zendy

Jan Huisken | Zendy; Didier Y. R. Stainier | Zendy

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Selective plane illumination microscopy techniques in developmental biology

Author(s) -

Jan Huisken,

Didier Y. R. Stainier

Publication year - 2009

Publication title -

development

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.754

H-Index - 325

eISSN - 1477-9129

pISSN - 0950-1991

DOI - 10.1242/dev.022426

Subject(s) - light sheet fluorescence microscopy , microscopy , fluorescence microscope , confocal microscopy , biology , photoactivated localization microscopy , optical sectioning , bright field microscopy , optical microscope , live cell imaging , fluorescence , optics , super resolution microscopy , scanning confocal electron microscopy , microbiology and biotechnology , scanning electron microscope , physics , genetics , cell

Selective plane illumination microscopy (SPIM) and other fluorescence microscopy techniques in which a focused sheet of light serves to illuminate the sample have become increasingly popular in developmental studies. Fluorescence light-sheet microscopy bridges the gap in image quality between fluorescence stereomicroscopy and high-resolution imaging of fixed tissue sections. In addition, high depth penetration, low bleaching and high acquisition speeds make light-sheet microscopy ideally suited for extended time-lapse experiments in live embryos. This review compares the benefits and challenges of light-sheet microscopy with established fluorescence microscopy techniques such as confocal microscopy and discusses the different implementations and applications of this easily adaptable technology.

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