Open AccessPreparation, characterisation and anti‐tumour activity of Ganoderma lucidum polysaccharide nanoparticles
Author(s) -
Li Ni,
Hu YuLan,
He CaiXia,
Hu ChengJie,
Zhou Jun,
Tang GuPing,
Gao JianQing
Publication year - 2010
Publication title -
journal of pharmacy and pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 118
eISSN - 2042-7158
pISSN - 0022-3573
DOI - 10.1211/jpp.62.01.0016
Subject(s) - hela , polysaccharide , cytotoxic t cell , cytotoxicity , chemistry , ganoderma lucidum , mtt assay , chitosan , spleen , in vitro , nanoparticle , biochemistry , pharmacology , immunology , biology , nanotechnology , materials science , food science
Objectives The aim was to prepare novel Ganoderma lucidum polysaccharide nanoparticles and to evaluate the physicochemical properties and anti‐tumour activity in in‐vitro cytotoxicity studies using HepG2, HeLa and A549 cancer cell lines, and growth promotion effects on mouse spleen cells. Methods Chitosan nanoparticles loaded with G. lucidum polysaccharide were prepared using the ion‐revulsion method. The diameter distribution of the particles and the surface charge were measured using a zetasizer analyser. The entrapment efficiency and drug loading capacity were examined by the diethylaminoethanol weak anion exchange method. The cytotoxic effects of nanoparticles on tumour cells and the growth promotion effects on mouse spleen cells were tested using the MTT assay. Key findings Nanoparticles loaded with G. lucidum polysaccharide at 6 μg/ml and chitosan/sodium tripolyphosphate (mass) ratio of 5.5 had significantly greater cytotoxic effects on tumour cells and growth promotion effects on mouse spleen cells than empty nanoparticles. Conclusions G. lucidum polysaccharide nanoparticles showed significant anti‐tumour efficacy, having both cytotoxic effects on tumour cells and growth promotion effects on spleen cells, making it a promising candidate in the clinical setting.