Open AccessInvestigations on cellular interaction of polyelectrolyte based nano‐walled reservoir using MCF‐7 cell lines: a novel chemotherapeutic approach
Author(s) -
Gupta Girish K.,
Gupta Vivek K.,
Shukla Prashant,
Pant Aditya B.,
Mishra Prabhat R.
Publication year - 2009
Publication title -
journal of pharmacy and pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 118
eISSN - 2042-7158
pISSN - 0022-3573
DOI - 10.1211/jpp.61.12.0003
Subject(s) - polyelectrolyte , pegylation , paclitaxel , chemistry , biophysics , surface modification , polyethylene glycol , nano , chemical engineering , materials science , nanotechnology , organic chemistry , polymer , cancer , biology , engineering , genetics
Objectives A polyelectrolyte (PE) based nano‐walled reservoir (NwR) was developed using alternate deposition of natural polyions on a decomposable core (CaCO 3 ). The system was charged with paclitaxel (PTX) using the trigger property of an organic solvent (NwR‐PTX). In addition, the surface of the nano‐walled reservoir was modified with PE‐PEG2000 (NwR‐PTX‐PEG)) in order to investigate any changes in the interaction of surface‐modified polyelectrolyte shells with breast cancer cells, since surface chemistry greatly influences the performance of microcapsules in the biological environment. Methods The surface modification was confirmed by differential scanning calorimetry studies, which showed a shifting of the endothermic peak after pegylation. Layer‐by‐layer (LBL) growth of the system was confirmed by the sequential change in the ζ‐potential. The release of paclitaxel from the formulations followed first order kinetics ( r 2 = 0.9), indicating matrix diffusion. The interaction of NwR‐PTX with MCF‐7 cell lines was investigated by coating the system with FITC‐dextran (NwR‐PTX‐FITC) and quantitated using flow cytometry. Key findings Cellular uptake of positively charged NwR reached 56% after 4 h and 76% after 24 h. This was reduced significantly after pegylation. The negatively charged NwR reached only 49% after 24 h. Conclusions This study opens the possibility of specific targeting of tumour cells that can control the release of chemotherapeutic agent either by means of a physiological or chemical trigger. This suggests potential application of this system as a novel approach for the delivery of chemotherapeutic agents.