Open AccessIn‐vitro and in‐vivo anti‐inflammatory action of the ethanol extract of Trachelospermi caulis
Author(s) -
Lee Mu Hong,
Lee Jeong Min,
Jun Sung Hoon,
Ha Chul Gyu,
Lee SeungHa,
Kim Nam Wook,
Lee Jun Ho,
Ko Na Young,
Mun Se Hwan,
Park Seung Hwa,
Kim Bo Kyung,
Her Erk,
Kim Young Mi,
Choi Wahn Soo
Publication year - 2007
Publication title -
journal of pharmacy and pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 118
eISSN - 2042-7158
pISSN - 0022-3573
DOI - 10.1211/jpp.59.1.0016
Subject(s) - in vivo , pharmacology , in vitro , traditional medicine , ethanol , chemistry , anti inflammatory , medicine , biology , biochemistry , microbiology and biotechnology
In this study, we aimed to investigate the anti‐inflammatory activity, antinociceptive activity and the action mechanism of Trachelospermi caulis extract. The anti‐inflammatory effects were investigated using arachidonic acid, 12‐ O ‐tetradecanoylphorbol 13‐acetate or carrageenan‐induced oedema assays. Antinociceptive activity, using the acetic acid‐induced writhing model, was also tested in mice. The extract exhibited dose‐dependent and significant ( P & 0.05 at 100–400 mg kg −1 ) anti‐inflammatory and antinociceptive activity in the animals. To further understand the mechanism of activity, we investigated whether the extract inhibited the expression of inducible nitric oxide synthase (iNOS), the production of nitric oxide (NO) and the expression of TNF‐α from murine macrophage RAW 264.7 cells. Similar to the in‐vivo activity, the iNOS expression, NO production and TNF‐α expression were found to be dose dependent and significantly suppressed by the extract through the inhibition of the p38 MAP kinase/NF‐κB pathway. Taken together, the results presented here suggest that T. caulis extract may be useful for the treatment of various inflammatory diseases.