Open AccessAttenuation of ciclosporin‐induced nephrotoxicity by dietary supplementation of seal oil in Sprague‐Dawley rats
Author(s) -
Yang Wei,
Kang Zhili,
Wang Lili,
Kara Mohamedtaki,
Herzberg Gene R.,
Robb Desmond,
Randell Edward,
Smeda John,
Xiong Jieying,
Liu Hu
Publication year - 2005
Publication title -
journal of pharmacy and pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 118
eISSN - 2042-7158
pISSN - 0022-3573
DOI - 10.1211/jpp.57.11.0015
Subject(s) - nephrotoxicity , ciclosporin , fish oil , polyunsaturated fatty acid , sunflower oil , blood urea nitrogen , chemistry , creatinine , lipid peroxidation , endocrinology , medicine , pharmacology , kidney , fatty acid , biochemistry , antioxidant , biology , fishery , fish <actinopterygii>
Fish oil, rich in omega‐3 (n‐3) polyunsaturated fatty acids (PUFAs), has been reported to attenuate nephrotoxicity induced by ciclosporin (cyclosporine A). Harp seal oil is a rich source of n‐3 PUFAs. This study investigated the ability of dietary seal oil to reduce nephrotoxicity caused by ciclosporin. Sprague‐Dawley rats were maintained on a standard diet (with sunflower oil as lipid, SFO) or a diet enriched with seal oil (with 85% seal oil and 15% sunflower oil as lipid, SO) for four weeks before and four weeks after intravenous administration of ciclosporin (15 mg kg −1 daily). Kidney function was assessed by measuring blood urea nitrogen, creatinine clearance, urinary N ‐acetyl‐1‐β‐ d ‐glucosaminidase, 6‐keto‐prostaglandin F 1α , thromboxane B 2 and malondialdehyde. Systolic blood pressure (SBP) was monitored. Ciclosporin concentrations in blood were measured using liquid chromatography‐tandem mass spectrometry (LC‐MS/MS). The fatty acid compositions of the diets and erythrocyte membranes were analysed by gas chromatography (GC). The results showed that nephrotoxicity was induced by ciclosporin in rats maintained on both SO and SFO diets. However, rats fed on SO diet endured less toxicity than those on SFO diet. The n‐3 and n‐6 PUFAs in the erythrocyte membrane of rats maintained on SO diet were found to be 10.79% and 11.93%, while those in rats maintained on SFO diet were found to be 1.67% and 22.71%, respectively. In conclusion, dietary supplementation of seal oil was found to reduce ciclosporin‐induced nephrotoxicity in rats.