Open AccessAbsorption characteristics of model compounds from the small intestinal serosal surface and a comparison with other organ surfaces
Author(s) -
Nishida Koyo,
Kuma Akiko,
Fumoto Shintaro,
Nakashima Mikiro,
Sasaki Hitoshi,
Nakamura Junzo
Publication year - 2005
Publication title -
journal of pharmacy and pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 118
eISSN - 2042-7158
pISSN - 0022-3573
DOI - 10.1211/0022357056677
Subject(s) - absorption (acoustics) , peritoneal cavity , small intestine , chemistry , kinetics , fluorescein isothiocyanate , materials science , biochemistry , anatomy , biology , fluorescence , physics , quantum mechanics , composite material
We examined the absorption of phenolsulfonphthalein (PSP) and fluorescein isothiocyanate dextrans (FD‐4, MW 4400; FD‐10, MW 9500; FD‐40, MW 40500) as model compounds through the small intestinal serosal surface. After application to the rat small intestinal serosal surface using a cylindrical diffusion cell, each compound was absorbed at different rates. The absorption ratios in 6 h after PSP, FD‐4, FD‐10 and FD‐40 application were calculated to be 89.2, 34.6, 14.9 and 2.1% of dose, respectively. Elimination profiles of PSP, FD‐4 and FD‐10 from the small intestinal serosal surface obeyed first‐order kinetics. Moreover, we calculated the apparent permeability coefficient P app for comparison to other organ surfaces. The kidney had the highest absorption efficiency, as shown by having more than 1.5 times significantly higher P app values of PSP, FD‐4 and FD‐10. Similar to the other organ surfaces, a correlation was observed between the P app of the small intestine and the molecular weight of these hydrophilic compounds. In addition, the small intestine is likely to contribute largely to hydrophilic compound absorption from the peritoneal cavity, judging from absorption clearance, CL a , calculated using the peritoneal organ surface area.