Open AccessInduction of apoptosis in human breast adenocarcinoma MCF‐7 cells by prodelphinidin B‐2 3,3′‐di‐ O ‐gallate from Myrica rubra via Fas‐mediated pathway
Author(s) -
Kuo PoLin,
Hsu YaLing,
Lin TaChen,
Lin LiangTzung,
Lin ChunChing
Publication year - 2004
Publication title -
journal of pharmacy and pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 118
eISSN - 2042-7158
pISSN - 0022-3573
DOI - 10.1211/0022357044625
Subject(s) - apoptosis , fas ligand , mcf 7 , gallate , myrica rubra , cancer cell , biology , chemistry , cancer research , programmed cell death , microbiology and biotechnology , cancer , biochemistry , pharmacology , human breast , botany , genetics
Abstract Myrica rubra Sieb et Zucc. (Myricaceae) is well known as a rich source of tannins. Prodelphinidin B‐2 3,3′‐di‐ O ‐gallate (PB233′OG) is a proanthocyanidin gallate that has been reported to exhibit anti‐oxidant and antiviral activity. In this study, we evaluated the anti‐proliferative activity of PB233′OG isolated from the bark of M. rubra in human breast adenocarcinoma MCF‐7 cells. To identity the anti‐cancer mechanism of PB233′OG, we assayed its effect on apoptosis, cell cycle distribution, and levels of p53, p21/WAF1, Fas/APO‐1 receptor and Fas ligand. The results showed that PB233′OG induced apoptosis of MCF‐7 cells without mediation of p53 and p21/WAF1. We suggest that Fas/Fas ligand apoptotic system is the main pathway of PB233′OG‐mediated apoptosis of MCF‐7 cells. Our study reports here for the first time that the activity of the Fas/Fas ligand apoptotic system may participate in the anti‐proliferative activity of PB233′OG in MCF‐7 cells.