Impairment of the renal p ‐aminohippurate transport induced by 6‐hydroxydopamine

In this study, the effects of 6‐hydroxydopamine (6‐OHDA) on renal p ‐aminohippurate transport were investigated in‐vitro using rat renal cortical slices. Cisplatin, a known nephrotoxin, was used as positive control. Renal cortical slices were incubated for 60 min in a cisplatin‐containing medium (0.83–5.0 μ m ) at 37°C under a 100% O 2 atmosphere. In another series of experiments, renal cortical slices were incubated in a 3.33 μ m cisplatin‐containing medium for 15–120min or in a cisplatin‐free medium. Subsequently, for each series of experiments, kidney slices were incubated at 25°C for 90 min in a media containing p ‐aminohippurate. In a further series of experiments, renal cortical slices were incubated for 60 min in a 6‐OHDA containing medium (3.125–100 μ m ) at 37°C under a 100% O 2 atmosphere. In another series of experiments, renal cortical slices were incubated in a 50 μ m 6‐OHDA‐containing medium for 15–120min or in 6‐OHDA‐free medium. Subsequently, for each series of experiments, kidney slices were incubated at 25°C for 90 min in a media containing p ‐aminohippurate. The results of this study where slices were incubated in 6‐OHDA‐ or cisplatin‐containing media indicate that both 6‐OHDA and cisplatin induced a time‐ and concentration‐dependent decrease in p ‐aminohippurate accumulation by renal cortical slices. Therefore, similarly to cisplatin, 6‐OHDA causes functional injury of renal proximal tubule cells, leading to impairment of transport processes across the cell membrane.