Open AccessAspirin and sodium salicylate inhibit proliferation and induce apoptosis in rheumatoid synovial cells
Author(s) -
Yamazaki Ryuta,
Kusunoki Natsuko,
Matsuzaki Takeshi,
Hashimoto Shusuke,
Kawai Shinichi
Publication year - 2002
Publication title -
journal of pharmacy and pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 118
eISSN - 2042-7158
pISSN - 0022-3573
DOI - 10.1211/002235702261
Subject(s) - sodium salicylate , tunel assay , dna fragmentation , apoptosis , viability assay , microbiology and biotechnology , chemistry , cell growth , fragmentation (computing) , terminal deoxynucleotidyl transferase , aspirin , pharmacology , immunology , medicine , biochemistry , biology , programmed cell death , ecology
Aspirin has been reported to induce apoptosis in a variety of cell lines. In this study, we examined whether aspirin and sodium salicylate inhibit cell growth and induce apoptosis in rheumatoid synovial cells. Synovial cells were obtained from patients with rheumatoid arthritis, and the cells were treated with aspirin or sodium salicylate (0.1–10 m m ) for 24 h. Cell proliferation and viability were assessed by 5‐bromo‐2′‐deoxyuridine incorporation and by 4‐[3‐(4‐iodophenyl)‐2‐(4‐nitrophenyl)‐ 2H ‐5‐tetrazolio]‐1,3‐benzene disulfonate (WST‐1) assay, respectively. The apoptosis of synovial cells was identified by DNA fragmentation assay and terminal deoxynucleotidyl transferase‐mediated dUTP nick end labelling (TUNEL) assay. Aspirin and sodium salicylate suppressed the proliferation (IC50 (concentration causing 50% inhibition of cell proliferation): 2.1 and 1.2 m m , respectively) and reduced the viability (IC50: 2.0 and 1.4 m m respectively) of synovial cells in a concentration‐dependent manner at 0.3–10 m m . Furthermore, they induced DNA fragmentation and increased the number of TUNEL‐ positive synovial cells. These results suggest that aspirin and sodium salicylate can inhibit the proliferation of rheumatoid synovial cells through induction of apoptosis.