Open AccessSequences Essential for Activity of the Thyroid Hormone Responsive Transcription Stimulatory Element of the Rat Growth Hormone Gene
Author(s) -
P.A.L. Wight,
Mark D. Crew,
Stephen R. Spindler
Publication year - 1988
Publication title -
molecular endocrinology
Language(s) - English
Resource type - Journals
eISSN - 1944-9917
pISSN - 0888-8809
DOI - 10.1210/mend-2-6-536
Subject(s) - biology , promoter activity , gene , transcription (linguistics) , hormone , microbiology and biotechnology , hormone response element , transfection , growth hormone , promoter , transcriptional activity , response element , thymidine kinase , transcription factor , gene expression , endocrinology , genetics , virus , linguistics , philosophy , cancer , estrogen receptor , herpes simplex virus , breast cancer
Thyroid hormone dependent transcription stimulatory and inhibitory elements exist at the 5'-end of the rat GH (rGH) gene (TSE and TIE, respectively). In this study, the location of the sequences essential for TSE activity was examined using stably transfected GC cells. Because the TIE may influence TSE activity, we investigated TSE activity both on the rGH promoter, in the presence of the TIE, and on the viral thymidine kinase promoter, with the TIE deleted. The results of these studies indicate that the minimum sequences essential for TSE activity exist between positions -194 and -169 of the rGH gene.
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