Identification and Characterization of Novel Rat and Human Gonad-Specific Organic Anion Transporters | Zendy

Takehiro Suzuki | Zendy; Tohru Onogawa | Zendy; Naoki Asano | Zendy; Hiroya Mizutamari | Zendy; Tsuyoshi Mikkaichi | Zendy; Masayuki Tanemoto | Zendy; Michiaki Abe | Zendy; Fumitoshi Satoh | Zendy; Michiaki Unno | Zendy; Kazuo Nunoki | Zendy; Masanori Suzuki | Zendy; Takanori Hishinuma | Zendy; Junichi Goto | Zendy; Tooru Shimosegawa | Zendy; Seiki Matsuno | Zendy; Sadayoshi Ito | Zendy; Takaaki Abe | Zendy

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Identification and Characterization of Novel Rat and Human Gonad-Specific Organic Anion Transporters

Author(s) -

Takehiro Suzuki,

Tohru Onogawa,

Naoki Asano,

Hiroya Mizutamari,

Tsuyoshi Mikkaichi,

Masayuki Tanemoto,

Michiaki Abe,

Fumitoshi Satoh,

Michiaki Unno,

Kazuo Nunoki,

Masanori Suzuki,

Takanori Hishinuma,

Junichi Goto,

Tooru Shimosegawa,

Seiki Matsuno,

Sadayoshi Ito,

Takaaki Abe

Publication year - 2003

Publication title -

molecular endocrinology

Language(s) - English

Resource type - Journals

eISSN - 1944-9917

pISSN - 0888-8809

DOI - 10.1210/me.2002-0304

Subject(s) - biology , xenopus , organic anion transporter 1 , gonad , microbiology and biotechnology , taurocholic acid , northern blot , epididymis , in situ hybridization , amino acid , sertoli cell , dehydroepiandrosterone , medicine , endocrinology , transporter , biochemistry , spermatogenesis , gene expression , hormone , gene , sperm , androgen , bile acid , botany

We have isolated three novel organic anion transporter cDNAs designated rat GST-1 (gonad-specific transporter), rat GST-2, and human GST, expressed at high levels in the testis. Rat GST-1, GST-2, and human GST consist of 748, 702, and 719 amino acids, respectively, and all molecules possess the 12 predicted transmembrane domains, which is a common structure of organic anion transporters. Northern blot analyses and in situ hybridization revealed that both of the rat molecules are highly expressed in the testis, especially in Sertoli cells, spermatogonia, and Leydig cells. Weak signals are also detected in the epididymis and ovary in adult rat. The exclusive expression of human GST mRNA in the testis was confirmed by RT-PCR. The pharmacological experiments of Xenopus laevis oocytes injected with the respective rat GST-1- and GST-2-cRNAs revealed that both rat GST-1 and GST-2 transport taurocholic acid, dehydroepiandrosterone sulfate, and T4 with Michaelis-Menten kinetics (taurocholic acid, Km = 8.9 and 2.5 microm, dehydroepiandrosterone sulfate, Km = 25.5 and 21.microm, and T4, Km = 6.4 and 5.8 for rat GST-1 and GST-2, respectively). T3 was also transported by rat GST-1 and GST-2. These data suggest that rat GST-1 and GST-2 might be one of the molecular entities responsible for transporting dehydroepiandrosterone sulfate and thyroid hormones involved in the regulation of sex steroid transportation and spermatogenesis in the gonad.

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