Expression of Melanin-Concentrating Hormone Receptor Messenger Ribonucleic Acid in Tumor Tissues of Pheochromocytoma, Ganglioneuroblastoma, and Neuroblastoma1 | Zendy

Kazuhiro Takahashi | Zendy; Kazuhito Totsune | Zendy; Osamu Murakami | Zendy; Masahiko Sone | Zendy; Fumitoshi Satoh | Zendy; Tomomi Kitamuro | Zendy; Takao Noshiro | Zendy; Yutaka Hayashi | Zendy; Hironobu Sasano | Zendy; Shigeki Shibahara | Zendy

Open Access

Expression of Melanin-Concentrating Hormone Receptor Messenger Ribonucleic Acid in Tumor Tissues of Pheochromocytoma, Ganglioneuroblastoma, and Neuroblastoma1

Author(s) -

Kazuhiro Takahashi,

Kazuhito Totsune,

Osamu Murakami,

Masahiko Sone,

Fumitoshi Satoh,

Tomomi Kitamuro,

Takao Noshiro,

Yutaka Hayashi,

Hironobu Sasano,

Shigeki Shibahara

Publication year - 2001

Publication title -

the journal of clinical endocrinology and metabolism

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.206

H-Index - 353

eISSN - 1945-7197

pISSN - 0021-972X

DOI - 10.1210/jcem.86.1.7158

Subject(s) - ganglioneuroblastoma , neuroblastoma , pheochromocytoma , biology , northern blot , messenger rna , endocrinology , adrenal medulla , pituitary tumors , medicine , receptor , cell culture , ganglioneuroma , catecholamine , gene , biochemistry , genetics

Expression of melanin-concentrating hormone (MCH) receptor messenger ribonucleic acid (mRNA) was studied by RT-PCR and Northern blot analysis in human brain; pituitary; adrenal glands; tumor tissues of adrenal tumors, ganglioneuroblastomas, and neuroblastomas; and various cultured tumor cell lines. RT-PCR analysis showed that MCH receptor mRNA was widely expressed in brain tissues, pituitary, normal portions of adrenal glands (cortex and medulla), tumor tissues of adrenocortical tumors (12 of 13 cases), pheochromocytoma (all 7 cases), ganglioneuroblastoma (1 case), neuroblastoma (all 5 cases), and various cultured tumor cell lines (6 of 7 cell lines), including 2 neuroblastoma cell lines. Northern blot analysis showed the expression of MCH receptor mRNA ( approximately 2.4 kb) only in the tumor tissues of 5 pheochromocytomas, 1 ganglioneuroblastoma, and 4 neuroblastomas, indicating that the expression levels of MCH receptor mRNA are much higher in these tumors than in the other tissues. These findings raised the possibility that MCH or MCH-like peptides may be related to the pathophysiology of these neural crest-derived tumors.

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