Liquid Chromatography-Mass Spectrometry Detection of Corticotropin-Releasing Hormone and Proopiomelanocortin-Derived Peptides in Human Skin1 | Zendy

Andrzej Słomiński | Zendy; Andrzej Szczesniewski | Zendy; Jacobo Wortsman | Zendy

Open Access

Liquid Chromatography-Mass Spectrometry Detection of Corticotropin-Releasing Hormone and Proopiomelanocortin-Derived Peptides in Human Skin¹

Author(s) -

Andrzej Słomiński,

Andrzej Szczesniewski,

Jacobo Wortsman

Publication year - 2000

Publication title -

the journal of clinical endocrinology and metabolism

Language(s) - English

Resource type - Journals

eISSN - 1945-7197

pISSN - 0021-972X

DOI - 10.1210/jcem.85.10.6863

Subject(s) - proopiomelanocortin , peptide , mass spectrometry , chemistry , peptide hormone , endogeny , prohormone , human skin , chromatography , liquid chromatography–mass spectrometry , corticotropin releasing hormone , hormone , endocrinology , medicine , biochemistry , biology , genetics

We have previously shown expression of CRH and POMC genes and peptides in the human skin. To ascertain the identity of those peptides, we used methods of peptide extraction and purification combined with the highly specific technique of liquid chromatography-mass spectrometry. Testing extracts of human skin, we identified endogenous peptides with masses and retention times corresponding to CRH, ACTH 1-39, ACTH 1-13, and alpha-MSH standards. Thus, conclusive evidence is provided for the presence of CRH and the POMC-derived ACTH 1-39, ACTH 1-13, and alpha-MSH peptides in human skin. Direct identification of these peptides is consistent with translation of the corresponding genes, and it also suggests intermediate pituitary lobe-like POMC peptide processing.

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