Retinoid Receptors Expression in Human Term Placenta: Involvement of RXRα in Retinoid Induced-hCG Secretion | Zendy

Jean Guibourdenche | Zendy; E. Alsat | Zendy; Fabrice Soncin | Zendy; Cécile RochetteEgly | Zendy; D. EvainBrion | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Retinoid Receptors Expression in Human Term Placenta: Involvement of RXRα in Retinoid Induced-hCG Secretion

Author(s) -

Jean Guibourdenche,

E. Alsat,

Fabrice Soncin,

Cécile RochetteEgly,

D. EvainBrion

Publication year - 1998

Publication title -

the journal of clinical endocrinology and metabolism

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.206

H-Index - 353

eISSN - 1945-7197

pISSN - 0021-972X

DOI - 10.1210/jcem.83.4.4860

Subject(s) - retinoid , retinoid x receptor , syncytiotrophoblast , retinoic acid , trophoblast , biology , receptor , endocrinology , secretion , placenta , in situ hybridization , medicine , microbiology and biotechnology , cell culture , fetus , gene expression , nuclear receptor , biochemistry , transcription factor , pregnancy , gene , genetics

To investigate the role of retinoids on human placental development and functions, we characterized the spatial distribution of retinoid receptors in human term chorionic villi. In situ hybridization with 35S labeled sense and antisense probes for the RARs, alpha, beta, gamma and RXRs, alpha, beta, gamma, specifically detected only RAR alpha and RXR alpha. Both RAR alpha and RXR alpha mRNA were preferentially expressed in the trophoblast cell layer. This syncytiotrophoblast expression was confirmed by immunohistochemical analyses using anti-RAR alpha and RXR alpha antibodies. Using trophoblast cells in culture, we then studied the effect on hCG secretion of 0.1 microM RA physiological forms and of selective RAR alpha and RXR alpha synthetic agonists. Only RXR alpha specific ligands such as physiological 9-cis RA and synthetic Ro 25-7386 stimulated hCG secretion (doubled). These results suggest an important role for RXR alpha in human placental development and function.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research