Open AccessCharacterization of Corticotropin-Releasing Hormone (CRH) in Human Skin1
Author(s) -
Andrzej Słomiński,
Gennady Ermak,
Joseph E. Mazurkiewicz,
James E. Baker,
Jacobo Wortsman
Publication year - 1998
Publication title -
the journal of clinical endocrinology and metabolism
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.206
H-Index - 353
eISSN - 1945-7197
pISSN - 0021-972X
DOI - 10.1210/jcem.83.3.4650
Subject(s) - hacat , endocrinology , medicine , forskolin , northern blot , corticotropin releasing hormone , messenger rna , receptor , biology , cell culture , urocortin , gene expression , downregulation and upregulation , human skin , hormone , stimulation , chemistry , gene , biochemistry , genetics
We have confirmed the expression of CRH and CRH receptor type 1 genes in human skin, cultured HaCaT keratinocytes, squamous cell carcinoma, and melanoma cells. The size of CRH messenger ribonucleic acid (mRNA), estimated by Northern blot hybridization, was 1.5 kilobases. CRH peptide was identified by reverse phase high pressure liquid chromatography separation in both whole skin and cultured cells. Forskolin and dexamethasone at concentrations of 10 micromol/L stimulated and inhibited, respectively, CRH peptide production in squamous cell carcinoma and melanoma cells, but had no significant effect on the CRH mRNA level. In melanoma cells, stimulation of melanogenesis down-regulated CRH receptor type 1 mRNA expression, but was without effect on CRH mRNA production. We suggest that in human skin the CRH signaling system is both operative and under regulatory control.
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