Open AccessHumanized Medium (h7H) Allows Long-Term Primary Follicular Thyroid Cultures From Human Normal Thyroid, Benign Neoplasm, and Cancer
Author(s) -
Susana B. Bravo,
María E.R. García-Rendueles,
Ángela R. Garcia-Rendueles,
Joana S. Rodrigues,
Sihara Pérez-Romero,
Montserrat García-Lavandeira,
Maria Suarez-Fariña,
Francisco Barreiro,
Barbara Czarnocka,
Ana Senra,
M.V. Lareu,
J. Rodríguez,
José CameselleTeijeiro,
Clara V. Álvarez
Publication year - 2013
Publication title -
the journal of clinical endocrinology and metabolism
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.206
H-Index - 353
eISSN - 1945-7197
pISSN - 0021-972X
DOI - 10.1210/jc.2012-3812
Subject(s) - thyroglobulin , thyroid , thyroid peroxidase , follicular cell , endocrinology , medicine , cell culture , biology , follicular phase , hormone , cancer research , genetics
Mechanisms of thyroid physiology and cancer are principally studied in follicular cell lines. However, human thyroid cancer lines were found to be heavily contaminated by other sources, and only one supposedly normal-thyroid cell line, immortalized with SV40 antigen, is available. In primary culture, human follicular cultures lose their phenotype after passage. We hypothesized that the loss of the thyroid phenotype could be related to culture conditions in which human cells are grown in medium optimized for rodent culture, including hormones with marked differences in its affinity for the relevant rodent/human receptor.
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