Free and Total Plasma Cortisol Measured by Immunoassay and Mass Spectrometry Following ACTH1–24 Stimulation in the Assessment of Pituitary Patients | Zendy

Morton G. Burt | Zendy; Brenda L. Mangelsdorf | Zendy; Anne Rogers | Zendy; Jui T. Ho | Zendy; John G. Lewis | Zendy; Warrick J. Inder | Zendy; Matthew Doogue | Zendy

Open Access

Free and Total Plasma Cortisol Measured by Immunoassay and Mass Spectrometry Following ACTH1–24 Stimulation in the Assessment of Pituitary Patients

Author(s) -

Morton G. Burt,

Brenda L. Mangelsdorf,

Anne Rogers,

Jui T. Ho,

John G. Lewis,

Warrick J. Inder,

Matthew Doogue

Publication year - 2013

Publication title -

the journal of clinical endocrinology and metabolism

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.206

H-Index - 353

eISSN - 1945-7197

pISSN - 0021-972X

DOI - 10.1210/jc.2012-3576

Subject(s) - immunoassay , medicine , endocrinology , adrenocorticotropic hormone , stimulation , chemistry , acth stimulation test , hydrocortisone , hormone , antibody , immunology

Measurement of plasma cortisol by immunoassay after ACTH₁₋₂₄ stimulation is used to assess the hypothalamic-pituitary-adrenal (HPA) axis. Liquid chromatography-tandem mass spectrometry (LCMS) has greater analytical specificity than immunoassay and equilibrium dialysis allows measurement of free plasma cortisol.

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