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Nighttime salivary cortisol (NSC) has been suggested to be a useful diagnostic test for Cushing's syndrome (CS). In the absence of published data on its day-to-day variability, we assessed the reproducibility of NSC by repeated measurements in healthy volunteers. Its diagnostic performance was compared with 24-h urinary free cortisol (UFC) and 1 mg overnight dexamethasone suppression test in 12 patients with CS, 20 healthy volunteers, 14 referred patients in which CS was excluded or not firmly established, 16 obese patients, and 20 women in late pregnancy.

the journal of clinical endocrinology and metabolism/journal of clinical endocrinology and metabolism

Reproducibility of Nighttime Salivary Cortisol and Its Use in the Diagnosis of Hypercortisolism Compared with Urinary Free Cortisol and Overnight Dexamethasone Suppression Test