Aurora B Overexpression Associates with the Thyroid Carcinoma Undifferentiated Phenotype and Is Required for Thyroid Carcinoma Cell Proliferation | Zendy

R Sorrentino | Zendy; Silvana Libertini | Zendy; Pier Lorenzo Pallante | Zendy; Giancarlo Troncone | Zendy; Lucio Palombini | Zendy; Vassilios Bavetsias | Zendy; Daniela Spalletti-Cernia | Zendy; Paolo Laccetti | Zendy; Spiros Linardopoulos | Zendy; Paolo Chieffi | Zendy; Alfredo Fusco | Zendy; Giuseppe Portella | Zendy

Open Access

Aurora B Overexpression Associates with the Thyroid Carcinoma Undifferentiated Phenotype and Is Required for Thyroid Carcinoma Cell Proliferation

Author(s) -

R Sorrentino,

Silvana Libertini,

Pier Lorenzo Pallante,

Giancarlo Troncone,

Lucio Palombini,

Vassilios Bavetsias,

Daniela Spalletti-Cernia,

Paolo Laccetti,

Spiros Linardopoulos,

Paolo Chieffi,

Alfredo Fusco,

Giuseppe Portella

Publication year - 2005

Publication title -

the journal of clinical endocrinology and metabolism

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.206

H-Index - 353

eISSN - 1945-7197

pISSN - 0021-972X

DOI - 10.1210/jc.2004-1518

Subject(s) - aurora b kinase , cancer research , biology , thyroid cancer , thyroid , thyroid carcinoma , mitosis , cell cycle , aurora inhibitor , aneuploidy , cancer , cell , cell division , chromosome , endocrinology , microbiology and biotechnology , spindle apparatus , gene , genetics

Alterations in chromosome number (aneuploidy) are common in human neoplasias. Loss of mitotic regulation is believed to induce aneuploidy in cancer cells and act as a driving force during the malignant progression. The serine/theronine protein kinases of aurora family genes play a critical role in the regulation of key cell cycle processes. Aurora B mediates chromosome segregation by ensuring orientation of sister chromatids and overexpression of Aurora B in diploid human cells NHDF (normal human diploid fibroblast) induces multinuclearity. We analyzed Aurora B expression in human thyroid carcinomas. Cell lines originating from different histotypes showed an increase in Aurora B expression. Immunohistochemical analysis of archive samples showed a high expression of Aurora B in anaplastic thyroid carcinomas; conversely, Aurora B expression was not detectable in normal thyroid tissue. Real-time PCR analysis confirmed a strong expression of Aurora B in anaplastic thyroid carcinomas. The block of Aurora B expression induced by RNA interference or by using an inhibitor of Aurora kinase activity significantly reduced the growth of thyroid anaplastic carcinoma cells.

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