Presence of Salmon Gonadotropin-Releasing Hormone (GnRH) and Compounds with GnRH-Like Activity in the Ovary of Goldfish1 | Zendy

Debananda Pati | Zendy; Hamid R. Habibi | Zendy

Open Access

Presence of Salmon Gonadotropin-Releasing Hormone (GnRH) and Compounds with GnRH-Like Activity in the Ovary of Goldfish¹

Author(s) -

Debananda Pati,

Hamid R. Habibi

Publication year - 1998

Publication title -

endocrinology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.674

H-Index - 257

eISSN - 1945-7170

pISSN - 0013-7227

DOI - 10.1210/endo.139.4.5877

Subject(s) - ovary , medicine , endocrinology , gonadotropin releasing hormone , gonadotropin , germinal vesicle , biology , follicle , antagonist , receptor , hormone , chemistry , oocyte , luteinizing hormone , embryo , microbiology and biotechnology

The presence of ovarian GnRH and/or compounds with GnRH-like activity was investigated in the goldfish ovary. Goldfish ovary was extracted using an acetone/HCl/ether mixture and was purified by Waters C-18 Sep-Pak columns (ovarian extract). The goldfish ovarian extract was found to 1) stimulate gonadotropin release and subunit messenger RNA production in the goldfish pituitary that was inhibited by a GnRH antagonist; 2) stimulate germinal vesicle breakdown in the prophase-I arrested follicle-enclosed goldfish oocytes in vitro, which was inhibited by a GnRH antagonist; 3) immunoreact with various GnRH antisera; and 4) bind to GnRH receptors in the goldfish pituitary and ovary as well as rat pituitary. Further purification with HPLC revealed the presence of two compounds with GnRH-like activity. One with identical chromatographic characteristics, amino acid composition, and primary structure to that of the salmon GnRH (sGnRH), and the other a novel compound with GnRH-like activity.

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