Expression and Effect of Adrenomedullin on Rat Granulosa Cell | Zendy

Kazuko Abe | Zendy; Takashi Minegishi | Zendy; Mari Tano | Zendy; Takashi Hirakawa | Zendy; Megumi Tsuchiya | Zendy; Kenji Kangawa | Zendy; Masayasu Kojima | Zendy; Yoshito Ibuki | Zendy

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Expression and Effect of Adrenomedullin on Rat Granulosa Cell

Author(s) -

Kazuko Abe,

Takashi Minegishi,

Mari Tano,

Takashi Hirakawa,

Megumi Tsuchiya,

Kenji Kangawa,

Masayasu Kojima,

Yoshito Ibuki

Publication year - 1998

Publication title -

endocrinology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.674

H-Index - 257

eISSN - 1945-7170

pISSN - 0013-7227

DOI - 10.1210/endo.139.12.6524

Subject(s) - adrenomedullin , endocrinology , medicine , autocrine signalling , paracrine signalling , receptor , granulosa cell , gonadotropin , messenger rna , northern blot , biology , follicle stimulating hormone receptor , intracellular , ovary , follicle stimulating hormone , hormone , luteinizing hormone , microbiology and biotechnology , gene , biochemistry

Adrenomedullin (AM) is a potent hypotensive peptide recently discovered in extracts of human pheochromocytoma. To elucidate the regulation of AM production in the ovary, the effect of gonadotropin on the production of AM was studied in the cultured rat granulosa cells. A Northern blot analysis of the LH receptor and adrenomedullin yielded a major hybridizing band at 5.4 kb and 1.6 kb, respectively. In our culture system of rat granulosa cells, without any stimulus, the LH receptor mRNA was undetectable and the AM mRNA level was stably expressed for 6 days. FSH significantly induced LH receptor mRNA and suppressed AM mRNA for 4 days of culture and with the addition of hCG after 2 days of pretreatment with FSH, AM mRNA levels were markedly suppressed. FSH and 8-Br-cAMP significantly increase LH receptor mRNA and suppress AM mRNA in a dose-dependent manner. These data indicated that the differentiation of granulosa cells mediated by gonadotropins were associated by suppression in AM expression through a cAMP-dependent mechanism. On the other hand, AM stimulated a rapid rise in intracellular cAMP levels, which peaked within 15 min of addition, in a dose-dependent manner with a maximal response seen at 100 nM. Additionally, AM enhanced the effects of FSH, acting additionally to produce cAMP in the cells. AM may play a role in the process of granulosa cell differentiation as a local regulator through an autocrine/paracrine mechanism.

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