Transgenic Expression of Enhanced Green Fluorescent Protein Enables Direct Visualization for Physiological Studies of Vasopressin Neurons and Isolated Nerve Terminals of the Rat | Zendy

Yoichi Ueta | Zendy; Hiroaki Fujihara | Zendy; Ryota Serino | Zendy; Govindan Dayanithi | Zendy; Hitoshi Ozawa | Zendy; Kenichi Matsuda | Zendy; Mitsuhiro Kawata | Zendy; Junko Yamada | Zendy; Shinya Ueno | Zendy; Atsuo Fukuda | Zendy; David Murphy | Zendy

Open Access

Transgenic Expression of Enhanced Green Fluorescent Protein Enables Direct Visualization for Physiological Studies of Vasopressin Neurons and Isolated Nerve Terminals of the Rat

Author(s) -

Yoichi Ueta,

Hiroaki Fujihara,

Ryota Serino,

Govindan Dayanithi,

Hitoshi Ozawa,

Kenichi Matsuda,

Mitsuhiro Kawata,

Junko Yamada,

Shinya Ueno,

Atsuo Fukuda,

David Murphy

Publication year - 2004

Publication title -

endocrinology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.674

H-Index - 257

eISSN - 1945-7170

pISSN - 0013-7227

DOI - 10.1210/en.2004-0830

Subject(s) - vasopressin , supraoptic nucleus , green fluorescent protein , suprachiasmatic nucleus , biology , medicine , axon , endocrinology , nucleus , transgene , microbiology and biotechnology , hypothalamus , neuropeptide , gene , receptor , biochemistry

We have generated transgenic rats expressing an arginine vasopressin (AVP)-enhanced green fluorescent protein (eGFP) fusion gene. The expression of the eGFP gene and strong fluorescence were observed in the supraoptic nucleus (SON), the paraventricular nucleus (PVN), and the suprachiasmatic nucleus (SCN) in transgenic rats. The hypothalamo-neurohypophyseal tract, isolated SON neurons, and isolated axon terminals in the neurohypophysis also showed robust eGFP fluorescence. Water deprivation for 2 d increased the fluorescence of the eGFP in the SON and the PVN but not the SCN. The whole-cell patch-clamp technique was then used to record the electrical activities specifically identifying eGFP-expressing SON, PVN, and SCN AVP neurons in in vitro brain slice preparations. The AVP-eGFP transgenic rats are a unique new tool with which to study the physiological role of AVP-secreting neurons in the central nervous system and the dynamics of the regulation of AVP secretion in the living neurons and their axon terminals.

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