Molecular Evidence of Organic Ion Transporters in the Rat Adrenal Cortex with Adrenocorticotropin-Regulated Zonal Expression | Zendy

Erzsébet Beéry | Zendy; Peter Middel | Zendy; Andrew Bahn | Zendy; Holger S. Willenberg | Zendy; Yohannes Hagos | Zendy; Hermann Koepsell | Zendy; SR Bornstein | Zendy; Gerhard A. Müller | Zendy; Gerhard Burckhardt | Zendy; J. Steffgen | Zendy

Open Access

Molecular Evidence of Organic Ion Transporters in the Rat Adrenal Cortex with Adrenocorticotropin-Regulated Zonal Expression

Author(s) -

Erzsébet Beéry,

Peter Middel,

Andrew Bahn,

Holger S. Willenberg,

Yohannes Hagos,

Hermann Koepsell,

SR Bornstein,

Gerhard A. Müller,

Gerhard Burckhardt,

J. Steffgen

Publication year - 2003

Publication title -

endocrinology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.674

H-Index - 257

eISSN - 1945-7170

pISSN - 0013-7227

DOI - 10.1210/en.2002-221001

Subject(s) - zona fasciculata , endocrinology , medicine , zona reticularis , zona glomerulosa , adrenal cortex , organic anion transporter 1 , median eminence , biology , aldosterone , corticosterone , chemistry , hormone , transporter , biochemistry , angiotensin ii , gene , blood pressure

Experimental evidence suggested that secretion of steroid hormones from adrenocortical cells involves carrier-mediated transport: Cortisol release from, and uptake of p-[3H]aminohippurate into, bovine adrenocortical cells showed properties of the renal p-[3H]aminohippurate/anion exchanger OAT1. Other poly-specific transporters such as organic anion-transporting polypeptides (oatps) and organic cation transporters (OCTs) could also be involved in steroid hormone release. A homology-cloning procedure was established to detect these transporters in rat adrenal gland cDNA. PCR revealed the presence of OAT1, oatp1, oatp2, and oatp3. In situ hybridization localized OAT1 in the outer zona fasciculata, oatp3 in the zona glomerulosa, and oatp1 and oatp2 in the inner zona fasciculata and outer zona reticularis. An OCT2-specific probe produced signals in the zona glomerulosa and outer zona fasciculata. Pretreatment of rats with ACTH increased the expression of OAT1 mRNA that spread to all zones, and hypophysectomy strongly decreased it. A less pronounced regulation was detected for OCT2 and oatp3. Specific antibodies confirmed the localization of OAT1 in the outer zona fasciculata, supporting a possible role of OAT1 in cortisol release. The zonated distribution of transporters furthermore suggest that oatp1-3 and OCT2 may be important for the endocrine function of rat adrenocortical cells.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research