On the limits of digital video microscopy | Zendy

Joerg Baumgartl | Zendy; Clemens Bechinger | Zendy

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On the limits of digital video microscopy

Author(s) -

Joerg Baumgartl,

Clemens Bechinger

Publication year - 2005

Publication title -

epl (europhysics letters)

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.625

H-Index - 165

eISSN - 1286-4854

pISSN - 0295-5075

DOI - 10.1209/epl/i2005-10107-2

Subject(s) - video microscopy , microscopy , digital video , computer science , computer graphics (images) , computer vision , materials science , physics , optics , telecommunications , biology , microbiology and biotechnology , transmission (telecommunications)

We explore the limits of digital video microscopy which is established as a standard method in physics, chemistry and biology. At particle distances close to contact we observe small but systematic deviations between the optically measured and the real particle distances. This difference is caused by the overlap of the optical images between neighboring particles. Exemplarily we discuss the consequences of this effect on pair potential measurements of charge stabilized colloids in confined geometries.

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