Open AccessPlatelet‐rich plasma does not reduce skeletal muscle fibrosis after distraction osteogenesis
Author(s) -
Tonogai Ichiro,
Hayashi Fumio,
Iwame Toshiyuki,
Takasago Tomoya,
Matsuura Tetsuya,
Sairyo Koichi
Publication year - 2018
Publication title -
journal of experimental orthopaedics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 18
ISSN - 2197-1153
DOI - 10.1186/s40634-018-0143-7
Subject(s) - distraction osteogenesis , platelet rich plasma , osteotomy , medicine , skeletal muscle , fibrosis , orthopedic surgery , bone healing , distraction , surgery , platelet , biology , neuroscience
Background Skeletal muscle fibrosis caused by an increase in collagen deposition often occurs after distraction osteogenesis. Although studies are available reporting the effects of platelet‐rich plasma (PRP) on tissue healing following injury, current findings remain controversial. This study focused on determining whether PRP reduces skeletal muscle fibrosis caused by distraction osteogenesis. Methods Tibial osteotomies were performed on 8‐week‐old wild type mice, and tibiae were distracted at a rate of 0.42 mm/day for 2 weeks, starting 1 week after osteotomy. Immediately after distraction was completed (3 weeks after osteotomy), PRP or phosphate buffered saline (as a sham) was injected into the gastrocnemius (GC) muscle. The GC muscles were harvested and analyzed. Results The amount and area of collagenous tissue increased in both the PRP and control groups following distraction osteogenesis, but the changes were not significantly different between both groups at all time points ( p = 0.89, 0.45, 0.33 and 0.52 at 4, 6, 8 and 10 weeks). Conclusion From this study, our results suggest that PRP did not significantly reduce skeletal muscle fibrosis due to distraction osteogenesis.