Open AccessEvaluation of Intraoperative Retention of Autologous Chondrocytes on Type I/III Collagen Scaffold (Ortho‐ACI™) for Cartilage Repair
Author(s) -
Crowe Raymond,
Willers Craig,
Cheng Taksum,
Wang Louis,
Zheng MingHao
Publication year - 2015
Publication title -
journal of foot and ankle research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.763
H-Index - 39
ISSN - 1757-1146
DOI - 10.1186/1757-1146-8-s2-p10
Subject(s) - medicine , aggrecan , scaffold , cartilage , type ii collagen , magnetic resonance imaging , chondrocyte , biomedical engineering , seeding , surgery , pathology , anatomy , osteoarthritis , articular cartilage , radiology , biology , agronomy , alternative medicine
Methods and Materials We tested cell retention on the scaffold by confocal microscopy at 7, 15, 20, 40, 60, 90 and 120 minutes after seeding, and the molecular profile (collagen II, aggrecan, Sox9, HAPLN1) of chondrocytes seeded at 20 minutes and 4 days (preoperative seeding method). Fifteen OrthoACITM patients with 25 cartilage defects were assessed by arthroscopic or magnetic resonance imaging (MRI). Graft repair was graded as excellent, good, poor or no infill. Associations between repair outcome and case variables were also investigated.