Uptake and Subcellular Distribution of Vitamin B12 in Mouse L1210 Leukemic Lymphoblasts | Zendy

E. M. Ryel | Zendy; Leo M. Meyer | Zendy; Richard A. Gams | Zendy

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Uptake and Subcellular Distribution of Vitamin B12 in Mouse L1210 Leukemic Lymphoblasts

Author(s) -

E. M. Ryel,

Leo M. Meyer,

Richard A. Gams

Publication year - 1974

Publication title -

blood

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.515

H-Index - 465

eISSN - 1528-0020

pISSN - 0006-4971

DOI - 10.1182/blood.v44.3.427.427

Subject(s) - vitamin b12 , lymphoblast , l1210 cells , cyanocobalamin , in vitro , biochemistry , cell fractionation , biology , chemistry , microbiology and biotechnology , cell culture , enzyme , cytotoxicity , genetics

Protein-mediated B12 uptake by L1210 lymphoblasts was shown to be calcium dependent and enhanced by TCII but not by TCI in vitro. Subcellular fractionation resulted in the majority of B12 localized in the soluble phase with significant amounts in the mitochondria. All vitamin B12 found in the soluble phase was bound to a protein similar in molecular weight to TCII. This protein was capable of delivering B12 to fresh L1210 cells.

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