INTERACTION OF ACIDIC AND BASIC DYES AND BEEF CORNEAL STROMA | Zendy

H.L. Kern | Zendy; W. Morton Grant | Zendy

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INTERACTION OF ACIDIC AND BASIC DYES AND BEEF CORNEAL STROMA

Author(s) -

H.L. Kern,

W. Morton Grant

Publication year - 1961

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/9.4.380

Subject(s) - mucoproteins , chemistry , methylene blue , stroma , biophysics , biochemistry , chromatography , biology , immunohistochemistry , immunology , photocatalysis , catalysis

Acidic and basic dyes react preferentially with the mucoproteins of beef corneal stroma. Free carboxyl groups of the mucoproteins can combine readily with cations; additional reaction with ions seems to involve an unmasking of charged groups in these proteins. Most of the dyes studied apparently react as aggregates in 0.02 M solution, and are fully dissociated only in much more dilute solution. The state of aggregation of methylene blue has a pronounced effect on the affinity with which this dye combines with the tissue, but the chemical reaction is limited, nevertheless, to the corneal mucoproteins.

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