RAPID PREPARATION OF FROZEN SECTIONS FOR COMPARATIVE HISTOCHEMICAL STUDIES: JUMP-MOUNTING OF PAIRED FROZEN SECTIONS ON SEMI-HEATED MICROSLIDES | Zendy

IAN I. IRONS | Zendy

Open Access

RAPID PREPARATION OF FROZEN SECTIONS FOR COMPARATIVE HISTOCHEMICAL STUDIES: JUMP-MOUNTING OF PAIRED FROZEN SECTIONS ON SEMI-HEATED MICROSLIDES

Author(s) -

IAN I. IRONS

Publication year - 1961

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/9.4.363

Subject(s) - microtome , frozen section procedure , tray , materials science , jump , biomedical engineering , chemistry , mechanical engineering , physics , surgery , optics , engineering , medicine , quantum mechanics

A technique for rapidly preparing a large number of paired and uniformly good quality frozen sections has been described in terms of specific procedures followed and apparatus required. Two innovations were noted: 1) a microtome chuck that allows precise alignment of two tissue blocks that are to be cut simultaneously, and 2) a heating tray for microslides that enables a controlled amount of heat to be applied to a small section of a slide that is subsequently used in "jump" mounting of two freshly cut sections.

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