THE HISTOCHEMICAL DEMONSTRATION OF α-d -GLUCOSIDASE IN MAMMALIAN TISSUES
Author(s) -
Alexander M. Rutenburg,
Julius A. Goldbarg,
S. H. Rutenburg,
RUTH T. LANG
Publication year - 1960
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/8.4.268
Subject(s) - duodenum , jejunum , enzyme , cortex (anatomy) , intestinal mucosa , ingestion , kidney , starvation , biology , chemistry , biochemistry , medicine , endocrinology , neuroscience
A histochemical method for the demonstration of α-d-glucosidase is presented. The procedure and the problems encountered in its development are discussed. Surveys of mammalian tissues showed that this enzyme is generally demonstrable by this method only in the mucosa of the duodenum and upper jejunum and in the kidney cortex. Starvation resulted in decreased enzymatic activity in the intestinal mucosa, whereas increased activity was observed after the ingestion of food.
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