CHROMATOGRAPHIC SEPARATION AND ISOLATION OF METACHROMATIC THIAZINE DYES
Author(s) -
Kenneth B. Taylor
Publication year - 1960
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/8.4.248
Subject(s) - metachromasia , alkyl , chromatography , thiazine , chemistry , solvent , absorption (acoustics) , chromatographic separation , organic chemistry , materials science , high performance liquid chromatography , biology , staining , genetics , composite material
1. The chromatographic behaviour of 22 closely related N-alkyl thionins in a number of solvent systems is described. 2. Details are given of the separation of commercial mixtures of the azures into their components and the application of this to the analysis of Romanowsky stains. 3. A table giving the R f equivalents and absorption maxima of the dyes is appended and the additive effect of alkyl substitution is discussed.
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