SOME OBSERVATIONS OF THE REACTION OF SCHIFF REAGENT WITH ALDEHYDES | Zendy

Tibor Barka | Zendy; L. S. Ornstein | Zendy

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SOME OBSERVATIONS OF THE REACTION OF SCHIFF REAGENT WITH ALDEHYDES

Author(s) -

Tibor Barka,

L. S. Ornstein

Publication year - 1960

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/8.3.208

Subject(s) - reagent , chemistry , feulgen stain , formaldehyde , aldehyde , chromatography , absorption (acoustics) , schiff base , nuclear chemistry , organic chemistry , polymer chemistry , biochemistry , materials science , catalysis , dna , composite material

From the reaction mixture of Schiff reagent and formaldehyde 3 to 6 (sometimes even 7) compounds can be separated on paper chromatogram. The Rf values of these compounds range from .09 to .74 and the absorption maxima from 535 to 574 mµ. The formation of these compounds is influenced by at least two factors: the SO 2 -content of the Schiff reagent and the molar ratio of aldehyde to Schiff reagent. Saturation of the Schiff reagent with SO 2 influences the hue of Feulgen stained nuclei and reduces the variation in Feulgen microspectrophotometry.

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