Book Reviews

140 Analytical Cytology. 2uuoi Ed. ROBERT C. I’ IELLoRs, En!. Mc-Crn_it Hill Book Co., New York, 1959. 534 pp. $17.50. The pnesenmt voitinue is devoted entirely to muew-er physic-al n_munI tO) n_ much less extent chemical methods of cytology. The book is divided into seven chapters : an entirely new one by Mellors on the immuonochemical n_Pl)roach with fluorescent tagged antigens and antibodies, the second, by Novikoff, also new’, attempts an iumtegrative synthesis of biochemistry n_nd morphology, the third by Barer of Oxford tnen_ts of phase, interference n_mud polarizinug microscopy in biology, the fourth by Selby treats of the soses of electron microscopy in cytology. Anne Engstr#{246}m gives us n_mmexcellent fifth chapter devoted to Xrn_y microscopy, Fitzgerald has contnibuteni the sixth chapter on n_utoradiography and the in_st chapter on photometricc-hemic-oil analysis of cells is contributed by Arthur Poihister and Leonard Ormmstein. I fotumud Mellon’s chapter most iliuminatimog. I)etn_ils n_re lucidly presented, so that the prepmorn_lion n_no! use of fluorescent antigens n_nd n_nmtibodies could readily be performed fronm his directions, n_nd enough of the reported usc-s of the proceduire n_re presented to give the studemot a very adequate appreciation of its value. Novikoff’s huuudred page comutnibutiouu is coniprehensive n_nd c-n_mmit-c-Il requoire a second and third ren_dimmg to fully appreciate its scope. Ultrn_cc-ntmifugationu separation of fractions, staining methods for e mzymes mm situ alud a variety of other appron_ches n_ne skillfully combined to give n_ui integrmoted pict tire of biological processes. The statenienit thoit the Millono renoctiomi demonstratenl both Iyrosiume noon! tryptophamm is perhaps misleadiumg in that it nitues mmot nientiomm that ultraviolet observn_t ionm is reottoireti for the tryptophamu react ion. Barer’s preseumtatiomm would be improved for mmmn_mmy puire niorphologists n_s well as histochemists, if some c-olmucretcn_pplic-atioums of the mn_thematic-al fomnmuiations were giveno. As originally conuceived, Ta Math .oara inc-ludeni all things discovered by oh)senvation, not their enumeration and d!uantitative rein_tiomoships alnumme (/hawO#{224}n’av, to find out by observatiolmu or i mmquimy). Nevertheless these procedures have a very couusidc-rable and real value iuu histochemmuistry in affording meamus of oienmomostratinmg the commtimmued presence of momphologicemmtities which hnove failed tou yield a chroniogc-mmicreact ioum. I agree itith Sc-Iby that. it-cn_ne only at the begiomnimug of the application of specific histochenmical and histoenzymn_tic ren_ctiomus iii electron microscopy n_mid am pensommahly quite hopeful of conutimmual progress in this directitun. The chapter presents a gooni view of tc-chmuical problems still existing in this field, n_mud should be a valuiable reference. I wouold not agree that the periodicac-in! Schiff reaction, alone, adequately identifies