SPECIFICITY OF LABELLED THYMIDINE AS A DEOXYRIBONUCLEIC ACID PRECURSOR IN RADIOAUTOGRAPHY | Zendy

Manabu Amano | Zendy; B. Messier | Zendy; C. P. Leblond | Zendy

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SPECIFICITY OF LABELLED THYMIDINE AS A DEOXYRIBONUCLEIC ACID PRECURSOR IN RADIOAUTOGRAPHY

Author(s) -

Manabu Amano,

B. Messier,

C. P. Leblond

Publication year - 1959

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/7.3.153

Subject(s) - thymidine , dna , biochemistry , chemistry , desoxyribonucleic acid , thymine , deoxyribonuclease , enzyme , nucleic acid , microbiology and biotechnology , biology

A simple method has been devised for the extraction of deoxyribonucleic acid from histological sections by means of the enzyme DNAse without significant loss of ribonucleic acid. Removal of deoxyribonucleic acid by this method completely eliminated the radioautographic reactions observed over nuclei after injection of thymidine-H 3 . It is concluded that labelled thymidine is an adequate tracer of newly formed deoxyribonucleic acid in radioautographs.

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