Open AccessHISTOCHEMICAL INVESTIGATIONS OF A SILVER METHOD FOR AXONS
Author(s) -
R.K. Winkelmann,
Robert W. Schmit
Publication year - 1959
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/7.2.86
Subject(s) - fixative , silver stain , amine gas treating , chemistry , sulfite , copper , reducing agent , silver nitrate , inorganic chemistry , solubility , silver nanoparticle , ion , substrate (aquarium) , silver oxide , nuclear chemistry , materials science , nanotechnology , organic chemistry , biochemistry , biology , nanoparticle , ecology , microbiology and biotechnology , cytoplasm
Silver ion is not specific either in location or in action in the present silver method. Studies with radioactive silver, with the dark-field microscope, and with simple reducing solutions demonstrate a general distribution of silver ion in the impregnated tissue. Copper and mercury may be substituted for silver in the impregnating solution. A water-soluble aldehyde is necessary as a fixative. The substrate for staining appears to require the presence of protein-bound amine groups. The product-forming solution does not require the presence of a reducing agent. Sulfite ion is not necessary. All controllable sources of base that were utilized produced the histologic results. The product appears to be silver oxide in terms of the present solubility studies.
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