DETERMINATION OF SUCCINIC DEHYDROGENASE ACTIVITY IN TISSUE CULTURE | Zendy

Nathan Kaufman | Zendy; Robert D. Hill | Zendy

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DETERMINATION OF SUCCINIC DEHYDROGENASE ACTIVITY IN TISSUE CULTURE

Author(s) -

Nathan Kaufman,

Robert D. Hill

Publication year - 1959

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/7.2.144

Subject(s) - incubation , substrate (aquarium) , agar , hela , dehydrogenase , succinic dehydrogenase , chemistry , biochemistry , succinate dehydrogenase , succinic acid , enzyme , chromatography , biology , cell , bacteria , ecology , genetics

Two methods of demonstrating succinic dehydrogenase activity in tissue cultures of HeLa cells have been demonstrated. One method employs a protective layer of agar applied to the cells prior to incubation in the selected substrate. The other method uses a mixture of agar and substrate which is placed on the cells; incubation follows without the further addition of substrate.

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