EVALUATION OF INDOPHENOL DERIVATIVES AS REAGENTS FOR DEMONSTRATING CYTOCHROME OXIDASE IN TISSUE SECTIONS | Zendy

David T. Crawford | Zendy; Marvin M. Nachlas | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

EVALUATION OF INDOPHENOL DERIVATIVES AS REAGENTS FOR DEMONSTRATING CYTOCHROME OXIDASE IN TISSUE SECTIONS

Author(s) -

David T. Crawford,

Marvin M. Nachlas

Publication year - 1958

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/6.6.438

Subject(s) - chemistry , cytochrome c oxidase , oxidase test , biochemistry , reagent , indophenol , enzyme , dehydrogenase , cytochrome , organic chemistry

Seven redox dyes were investigated as possible histochemical indicators of cytochrome oxidase activity. All failed to meet one prime requisite, namely, that the oxidized product remain at the site of enzymatic action. The "color fading" phenomenon was studied. This effect was one in which sections and homogenates became colored as a result of oxidase activity and then faded during further incubation. Evidence was provided that this reaction occurred as a result of endogenous dehydrogenase activity, mainly succinic dehydrogenase. The decoloration in solution could be prevented by N-ethyl maleimide, without inhibiting cytochrome oxidase.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research