THE SITE OF A DIFFUSIBLE NUCLEOTIDE REVEALED BY A COMPARISON OF CHEMICALLY FIXED TISSUE WITH FROZEN AND DRIED TISSUE
Author(s) -
Laura Bell
Publication year - 1958
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/6.6.435
Subject(s) - nucleotide , reagent , fixation (population genetics) , chemistry , biochemistry , chromatography , microbiology and biotechnology , biology , organic chemistry , gene
1. Using unfixed frozen and dried tissue as a standard it is shown that acid alcohol fixation extracts a diffusible nucleotide from the vicinity of the chromosomes. 2. It is shown that the denuded area contains protein and that the end groups are not available for reaction with protein reagents.
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