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1. Using unfixed frozen and dried tissue as a standard it is shown that acid alcohol fixation extracts a diffusible nucleotide from the vicinity of the chromosomes.    2. It is shown that the denuded area contains protein and that the end groups are not available for reaction with protein reagents.

THE SITE OF A DIFFUSIBLE NUCLEOTIDE REVEALED BY A COMPARISON OF CHEMICALLY FIXED TISSUE WITH FROZEN AND DRIED TISSUE