IMPROVED TECHNIQUE FOR THE HISTOCHEMICAL DEMONSTRATION OF SUCCINIC DEHYDROGENASE IN BRAIN TISSUE | Zendy

Reinhard L. Friede | Zendy

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IMPROVED TECHNIQUE FOR THE HISTOCHEMICAL DEMONSTRATION OF SUCCINIC DEHYDROGENASE IN BRAIN TISSUE

Author(s) -

Reinhard L. Friede

Publication year - 1958

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/6.5.347

Subject(s) - succinic dehydrogenase , brain tissue , succinate dehydrogenase , stain , lactic dehydrogenase , chemistry , dehydrogenase , biochemistry , enzyme , anatomy , staining , pathology , biology , medicine

A tissue cutting technique for the histochemical demonstration of succinic dehydrogenase activity in brain tissue is described. The demonstration of succinic dehydrogenase in fine and delicate structures depends on a tissue temperature of –3 to –2°C. during cutting. Artefacts such as coarse granules, loss of activity and diffusion of stain are prevented by minimizing of traumatic effects due to this improved technique.

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