HISTOCHEMICAL DEMONSTRATION OF BRANCHING ENZYME (AMYLO-1,4 →1,6-TRANSGLUCOSIDASE) IN ANIMAL TISSUES | Zendy

Tadao Takeuchi | Zendy

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HISTOCHEMICAL DEMONSTRATION OF BRANCHING ENZYME (AMYLO-1,4 →1,6-TRANSGLUCOSIDASE) IN ANIMAL TISSUES

Author(s) -

Tadao Takeuchi

Publication year - 1958

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/6.3.208

Subject(s) - enzyme , enzyme assay , epithelium , biology , skeletal muscle , biochemistry , anatomy , chemistry , pathology , medicine , genetics

The procedure for the histochemical demonstration of branching enzyme (amylo-1,4 → 1,6-transglucosidase) activity is given. Procedures to determine the reliability of the results of localization of enzymatic activity and the histochemical relation between branching enzyme and amylophosphorylase are discussed. The results in normal tissues of the rabbit are presented. The distribution of amylo-1,4 → 1,6-transglucosidase in animal tissue cells is quite similar to that of amylophosphorylase. The quantitative balance of each enzyme in tissue cells is changed respectively according to various conditions. Greatest concentration of enzymatic activity is seen in liver, skeletal muscle, heart muscle, smooth muscle of various organs, nervous tissues and epithelium of certain organs.

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