THE USEFULNESS OF THE BROMINATION VARIANT IN THE FEULGEN MICROSPECTROPHOTOMETRY
Author(s) -
Tibor Barka,
Gustav Dallner
Publication year - 1958
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/6.3.197
Subject(s) - feulgen stain , halogenation , staining , biochemistry , chemistry , biology , dna , organic chemistry , genetics
The bromination Feulgen variant (T. Barka: J. Histochem. Cytochem. 4: 208, 1956) can be used even in the Feulgen microspectrophotometry. The decrease in UV-absorption and gallocyanin-chromalum staining caused by brominatoin suggest that the bromination depolymerizes but one part of the ribonucleic acid, beside that it releases aldehyde groups in the deoxyribonucleic acid.
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