HISTOCHEMICAL DEMONSTRATION OF β-D-GALACTOSIDASE IN THE RAT | Zendy

Alexander M. Rutenburg | Zendy; S. H. Rutenburg | Zendy; Benito Monis | Zendy; RUTH TEAGUE | Zendy; Arnold M. Seligman | Zendy

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HISTOCHEMICAL DEMONSTRATION OF β-D-GALACTOSIDASE IN THE RAT

Author(s) -

Alexander M. Rutenburg,

S. H. Rutenburg,

Benito Monis,

RUTH TEAGUE,

Arnold M. Seligman

Publication year - 1958

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/6.2.122

Subject(s) - epididymis , vas deferens , thyroid , spleen , pancreas , connective tissue , pathology , cytoplasm , gastrointestinal tract , kidney , biology , lung , frozen section procedure , anatomy , medicine , endocrinology , microbiology and biotechnology , sperm , biochemistry , immunology , botany

A histochemical technic for demonstration of β-d-galactosidase in formalin fixed sections has been presented. Enzymatic activity was best demonstrated in frozen sections of the kidney, liver, gastrointestinal tract, testis, epididymis and vas deferens of the rat and to a lesser extent in salivary gland, pancreas, thyroid, trachea, lung, spleen and lymphoid tissue. Leucocytes stained intensely. Enzymatic activity was largely present in the cytoplasm of epithelial cells. Cell nuclei, myocardium, connective tissue and erythrocytes were inactive.

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